| Abstract: | Our goal is to develop an at-home COVID-19 testing kit composed of a sample pretreatment and a sandwich ELISA lateral paper flow testing strip that will yield results in 20 minutes. Our design utilizes an ELISA format, using an indication component (quantum dots) that is easily readable, and a detection component (antibodies) that is reliable. To achieve this goal, three specific aims are proposed: (1) Fabrication of a point-of-care lateral flow paper strip detection device, (2) Evaluate the performance of the design with patient samples (3) Adjusting design to add serology testing lines to reveal which stage of COVID-19 infection a patient is in. The main innovative aspects of the design are (1) a user-friendly setup that is affordable and readily available to the public, (2) uses a non-invasive sample source, (3) has detection principles that are easily adaptable to detect many different infections with minimal adjustments. The impact of this research will save the lives of millions as it would serve to alert users if they are infected and/or asymptomatic indicating whether they should quarantine or get early medical attention. |